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KMID : 0360220110520060671
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Journal of the Korean Ophthalmological Society
2011 Volume.52 No. 6 p.671 ~ p.678
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Effects of Mitomycin C on Scleral Collagen Fibrils According to Atomic Force Microscopy
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Lee Hui-Jae
Choi Sam-Jin
Cheong You-Jin
Jin Kyung-Hyun
Park Hun-Kuk
Lee Seung-Jun
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Abstract
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Purpose: To investigate the effects of mitomycin C on the scleral collagen surfaces using atomic force microscopy (AFM).
Methods: Two non-contact mode AFM machines were used to observe changes in the morphological characteristics of human scleral surfaces before and after one, three, and five minutes of 0.02% mitomycin C application. Based on AFM topography and deflection images of the collagen fibril, the morphological characteristics of scleral fibrils including the fibril diameter and D-period were measured using the line profile.
Results: The sclera collagen fibril treated with 0.02% mitomycin C for one minute did not show any significant increases in mean fibril diameter (155.04 ± 17.46 nm) or mean D-periodicity (70.02 ± 3.33 nm), compared to those of the control group. However, the scleral collagen fibrils treated with 0.02% mitomycin C for three and five minutes showed significant increases in mean fibril diameter (182.33 ± 16.33 nm, 199.20 ± 12.40 nm, respectively) and mean D-periodicity (70.27 ± 13.66 nm, 72.75 ± 19.32 nm, respectively), compared to those of the control group.
Conclusions: The present study examined the structural changes in the scleral collagen fibrils before and after mitomycin C application according to atomic force microscopy. The results indirectly suggest that three or more minutes of 0.02% mitomycin C application affects the morphology of scleral collagen.
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KEYWORD
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Atomic force microscopy, Collagen fibrils, Diameter, D-banding, Human sclera, Mitomycin C
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